Proteomics

Dataset Information

0

Serum Peptidome: Diagnostic Window into Pathogenic Processes following Occupational Exposure to Carbon Nanomaterials

ABSTRACT: Growing industrial use of carbon nanotubes and nanofibers (CNT/F) warrants consideration of human health outcomes. CNT/F produces pulmonary, cardiovascular, and other toxic effects in animals, with significant augmentation of bioactive circulating peptides, the serum peptidome. While epidemiology among CNT/F workers reports few acute symptoms, there remains concern that CNT/F may prime for chronic disease. Here, the serum peptidome is assessed for its biomarker potential in detecting sub-symptomatic pathobiology among CNT/F workers using label-free data-independent mass spectrometry. Using a stratified design between High (>0.5 ug/m3) and Low (<0.1 ug/m3) CNT/F exposures, 2,726 serum peptides significantly distinguish the groups. Of these, 41 are found to be highly discriminatory after model building and exhibit a strong linear correlation with personal CNT/F exposures. The top-five peptide model offers ideal prediction with high accuracy (Q2=0.99916). Unsupervised validation affirms 43.5% of the serum peptidomic variance is attributable to CNT/F exposure. Peptide sequence identification reveals a predominant association with vascular pathology. ARHGAP21, ADAM15 and PLPP3 peptides suggest heightened cardiovasculature permeability and F13A1, FBN1 and VWDE peptides infer a pro-thrombotic state among High CNT/F workers. The serum peptidome affords a diagnostic window into pre-symptomatic pathology among CNT/F exposed workers for longitudinal monitoring health risks.

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Andrew K Ottens  

PROVIDER: MSV000087305 | MassIVE | Mon Apr 26 11:55:00 BST 2021

SECONDARY ACCESSION(S): PXD025646

REPOSITORIES: MassIVE

Json Xml

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

Serum peptidome: diagnostic window into pathogenic processes following occupational exposure to carbon nanomaterials.

Mostovenko Ekaterina E   Dahm Matthew M MM   Schubauer-Berigan Mary K MK   Eye Tracy T   Erdely Aaron A   Young Tamara L TL   Campen Matthew J MJ   Ottens Andrew K AK  

Particle and fibre toxicology 20211028 1

<h4>Background</h4>Growing industrial use of carbon nanotubes and nanofibers (CNT/F) warrants consideration of human health outcomes. CNT/F produces pulmonary, cardiovascular, and other toxic effects in animals along with a significant release of bioactive peptides into the circulation, the augmented serum peptidome. While epidemiology among CNT/F workers reports on few acute symptoms, there remains concern over sub-clinical CNT/F effects that may prime for chronic disease, necessitating sensiti  ...[more]