Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF-X, Orbitrap Fusion, Q Exactive
ORGANISM(S): Mus Musculus (ncbitaxon:10090)
SUBMITTER: Benjamin A. Garcia
PROVIDER: MSV000087342 | MassIVE | Fri Apr 30 07:04:00 BST 2021
SECONDARY ACCESSION(S): PXD025742
REPOSITORIES: MassIVE
Baeza Josue J Coons Barbara E BE Lin Zongtao Z Riley John J Mendoza Mariel M Peranteau William H WH Garcia Benjamin A BA
Cell reports methods 20240201 2
Protein translational control is critical for ensuring that the fetus develops correctly and that necessary organs and tissues are formed and functional. We developed an in utero method to quantify tissue-specific protein dynamics by monitoring amino acid incorporation into the proteome after pulse injection. Fetuses of pregnant mice were injected with isotopically labeled lysine and arginine via the vitelline vein at various embyonic days, and organs and tissues were harvested. By analyzing the ...[more]