Proteomics

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An ultrasensitive method for analysis of viral spike N-glycoforms


ABSTRACT: Viruses can evade the host immune system by displaying numerous glycans on their surface "spike-proteins" that cover immune epitopes. We have developed an ultrasensitive "single pot" method to assess glycan occupancy and the extent of glycan processing from high-mannose to complex forms at each N-glycosylation site. Though aimed at characterizing glycosylation of viral spike proteins as potential vaccines, this method is applicable for analysis of site-specific glycosylation of any glycoprotein.

INSTRUMENT(S): timsTOF Pro, Q Exactive HF-X

ORGANISM(S): Bg505 Sosip.664 Md39 Bg505 Sosip.664 Homo Sapiens (ncbitaxon:9606)

SUBMITTER: John R. Yates III  

PROVIDER: MSV000087414 | MassIVE | Wed May 12 20:28:00 BST 2021

SECONDARY ACCESSION(S): PXD025990

REPOSITORIES: MassIVE

Dataset's files

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Publications

DeGlyPHER: An Ultrasensitive Method for the Analysis of Viral Spike <i>N</i>-Glycoforms.

Baboo Sabyasachi S   Diedrich Jolene K JK   Martínez-Bartolomé Salvador S   Wang Xiaoning X   Schiffner Torben T   Groschel Bettina B   Schief William R WR   Paulson James C JC   Yates John R JR  

Analytical chemistry 20211001 40


Viruses can evade the host immune system by displaying numerous glycans on their surface "spike-proteins" that cover immune epitopes. We have developed an ultrasensitive "single-pot" method to assess glycan occupancy and the extent of glycan processing from high-mannose to complex forms at each <i>N</i>-glycosylation site. Though aimed at characterizing glycosylation of viral spike-proteins as potential vaccines, this method is applicable for the analysis of site-specific glycosylation of any gl  ...[more]

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