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Supplemental metabolomics data files for "Hierarchical control of microbial community assembly"

ABSTRACT: This metabolomics data supplements "Hierarchical control of microbial community assembly" Sammy Pontrelli, Rachel Szabo, Shaul Pollak, Julia Schwartzman, Daniela Ledezma-Tejeida, Otto X. Cordero, Uwe Sauer Datasets include FIA-QTOF-MS and LC-MS data of metabolite secretion and consumption data of 18 marine bacterial isolates. Raw .d files are included, as well as metadata that describes each dataset.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Vibrio Sp. G2r10 Alteromonas Sp. A3r04 Citricella Sp. C3m06 Vibrio Sp. Vnigr-6d03 (ncbitaxon:2058088) Vibrio Sp. 1a01 (ncbitaxon:314742) Silicibacter Sp. A3r06 F__pseudoalteromonadaceae I2r16 Reinekea Sp. G2m07 Marinobacter Sp. F3r11 Phaeobacter Sp. B3m02 Amphritea Sp.c1r06 Psychromonas Sp. Psych-6c06 (ncbitaxon:2058089) Marinobacter Sp. F3r08 Paracoccus Kamogawaensis C2r09 Marinobacter Sp. D2m19 Vibrio Sp. C3r12 Colwellia Psychrerythraea C2m11

SUBMITTER: Uwe Sauer

PROVIDER: MSV000087455 | MassIVE | Tue May 18 23:42:00 BST 2021

REPOSITORIES: MassIVE

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Project description:Wastewater treatment plants (WWTPs) are host to diverse microbial communities and receive a constant influx of microbes from influent wastewater. However, the impact of immigrants on the structure and activities of the activated sludge (AS) microbial community remains unclear. To gain insight on this phenomenon known as perpetual community coalescence, the current study utilized controlled manipulative experiments that decoupled the influent wastewater composition from the microbial populations to reveal the fundamental mechanisms involved in immigration between sewers and AS-WWTP. The immigration dynamics of heterotrophs were analyzed by harvesting wastewater biomass solids from three different sewer systems and adding to synthetic wastewater. Immigrating influent populations were observed to contribute up to 14% of the sequencing reads in the AS. By modeling the net growth rate of taxa, it was revealed that immigrants primarily exhibited low or negative net growth rates. By developing a protocol to reproducibly grow AS-WWTP communities in the lab, we have laid down the foundational principles for the testing of operational factors creating community variations with low noise and appropriate replication. Understanding the processes that drive microbial community diversity and assembly is a key question in microbial ecology. In the future, this knowledge can be used to manipulate the structure of microbial communities and improve system performance in WWTPs.IMPORTANCEIn biological wastewater treatment processes, the microbial community composition is essential in the performance and stability of the system. This study developed a reproducible protocol to investigate the impact of influent immigration (or perpetual coalescence of the sewer and activated sludge communities) with appropriate reproducibility and controls, allowing intrinsic definitions of core and immigrant populations to be established. The method developed herein will allow sequential manipulative experiments to be performed to test specific hypothesis and optimize wastewater treatment processes to meet new treatment goals.

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Supplemental metabolomics data files for "Hierarchical control of microbial community assembly"

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