Dataset Information

Global ubiquitylation analysis of mitochondria in primary neurons following PINK1-dependent activation of Parkin

ABSTRACT: Dataset analyzed in "Global ubiquitylation analysis of mitochondria in primary neurons following PINK1-dependent activation of Parkin" paper. Experiment TMT-labeled neurons untreated vs 5h AO treatment: 1) Membrane Proteome: .raw files AO2445-AO2456 2) Ubiquitylome: .raw files AO2433-AO2438 Experiment TMT-labeled neurons wild-type vs PINK1 KO genotype, untreated vs 5h AO treatment: 1) Membrane Proteome: .raw files AO3009-AO3020 2) Ubiquitylome: .raw files AO2997-3002 Experiment AQUA-Ubiquitin - .raw file associated with Figure1CD: UT (AO1987, AO1989, AO1991) AO (AO1988, AO1990, AO1992) Experiment AQUA-Ubiquitin - .raw file associated with FigureS3A: UT (AO1777, AO1779, AO1781, AO1783) AO (AO1778, AO1780, AO1782, AO1784) Experiment AQUA-Ubiquitin - .raw file associated with FigureS3B: UT (AO1823, AO1825, AO1827, AO1829) AO (AO1824, AO1826, AO1828, AO1830)

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: J. Wade Harper Miratul Muqit

PROVIDER: MSV000087639 | MassIVE | Wed Jun 16 08:18:00 BST 2021

REPOSITORIES: MassIVE

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Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Antico Odetta O Ordureau Alban A Stevens Michael M Singh Francois F Nirujogi Raja S RS Gierlinski Marek M Barini Erica E Rickwood Mollie L ML Prescott Alan A Toth Rachel R Ganley Ian G IG Harper J Wade JW Muqit Miratul M K MMK

Science advances 20211112 46

How activation of PINK1 and Parkin leads to elimination of damaged mitochondria by mitophagy is largely based on cell lines with few studies in neurons. Here, we have undertaken proteomic analysis of mitochondria from mouse neurons to identify ubiquitylated substrates of endogenous Parkin. Comparative analysis with human iNeuron datasets revealed a subset of 49 PINK1 activation–dependent diGLY sites in 22 proteins conserved across mouse and human systems. We use reconstitution assays to demonstr ...[more]

PMID: 34767452

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