Gut microbiome cholesterol associations
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF, TSQ Quantis
ORGANISM(S): Lactobacillus Amylovorus (ncbitaxon:1604) Bacteroides Ovatus (ncbitaxon:28116) Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606) Ruminococcaceae (ncbitaxon:541000) Anaerostipes Caccae Dsm 14662 (ncbitaxon:411490) Bacteroides Vulgatus (ncbitaxon:821) Bacteroides Uniformis (ncbitaxon:820) Bifidobacterium Longum Subsp. Infantis Atcc 15697 = Jcm 1222 (ncbitaxon:391904) Bacteroides Thetaiotaomicron (ncbitaxon:818) Bacteroides Fragilis (ncbitaxon:817) Bifidobacterium Pseudolongum Subsp. Pseudolongum (ncbitaxon:31954) Mus Sp. (ncbitaxon:10095) Bacteroides Caccae (ncbitaxon:47678)
SUBMITTER: 
Elizabeth Johnson
PROVIDER: MSV000087688 | MassIVE | Wed Jun 23 09:24:00 BST 2021
REPOSITORIES: MassIVE
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Project description:Metabolomic analysis of cholesterol metabolism via the gut microbiome
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2012-06-30 | E-GEOD-38880 | biostudies-arrayexpress
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Project description:The gut microbiome is a malleable microbial community that can remodel in response to various factors, including diet, and contribute to the development of several chronic diseases, including atherosclerosis. We devised an in vitro screening protocol of the mouse gut microbiome to discover molecules that can selectively modify bacterial growth. This approach was used to identify cyclic D,L-α-peptides that remodeled the Western diet (WD) gut microbiome toward the low-fat-diet microbiome state. Daily oral administration of the peptides in WD-fed LDLr-/- mice reduced plasma total cholesterol levels and atherosclerotic plaques. Depletion of the microbiome with antibiotics abrogated these effects. Peptide treatment reprogrammed the microbiome transcriptome, suppressed the production of pro-inflammatory cytokines (including interleukin-6, tumor necrosis factor-α and interleukin-1β), rebalanced levels of short-chain fatty acids and bile acids, improved gut barrier integrity and increased intestinal T regulatory cells. Directed chemical manipulation provides an additional tool for deciphering the chemical biology of the gut microbiome and might advance microbiome-targeted therapeutics.
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