Proteomics

Dataset Information

0

The NuRD complex is required for Thpok-mediated CD4+ T cell development

ABSTRACT: This data represent characterization of the interactome of murine Thpok. A version of Thpok (Thpok-Bio) subject to biotinylation in vivo by the BirA biotin ligase was used; to assess Thpok interactions in primary cells, Thpok-Bio was retrovirally expressed in in vitro activated CD4+ T cells from Thpokfl/fl Ox40-Cre+ mice carrying a Rosa26BirA allele. Expression of Ox40-Cre, initiated upon T cell activation, causes the deletion of endogenous Thpokfl alleles, so that transduced cells only express Thpok-Bio. Two Thpok deletion constructs (BKK and RFK) were also analyzed. Following streptavidin pulldown, proteins were separated by SDS-PAGE, the full lane cut into 9 slices, and in-gel digested. FIles are: 1384 - empty vector; 1385 - Thpok; 1386 - Thpok + Ethidium Bromide; 1387 - Thpok BKK; 1388 - Thpok RFK.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Lisa Jenkins  

PROVIDER: MSV000088126 | MassIVE |

SECONDARY ACCESSION(S): PXD028661

REPOSITORIES: MassIVE

Json Xml

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

Although BTB-zinc finger (BTB-ZF) transcription factors control the differentiation of multiple hematopoietic and immune lineages, how they function is poorly understood. The BTB-ZF factor Thpok controls intrathymic CD4<sup>+</sup> T cell development and the expression of most CD4<sup>+</sup> and CD8<sup>+</sup> lineage genes. Here, we identify the nucleosome remodeling and deacetylase (NuRD) complex as a critical Thpok cofactor. Using mass spectrometry and coimmunoprecipitation in primary T cel  ...[more]

Similar Datasets

Transcriptomics Self-Enforcing Feedback Activation between BCL6 and Pre-B Cell Receptor Signaling Defines a Distinct Subtype of Acute Lymphoblastic Leukemia
2015-02-08 | GSE59332 | GEO
Transcriptomics PP2A mediates malignant pre-B cell survival through Lineage-Specific control of cellular redox homeostasis
2018-02-12 | GSE83742 | GEO
Transcriptomics Chronic active pre-B cell receptor signaling drives BCL6 expression and represents a therapeutic target in a distinct subtype of acute lymphoblastic leukemia
2015-02-08 | E-GEOD-59332 | biostudies-arrayexpress
Proteomics Decoupling astrocytes in adult mice impairs synaptic plasticity and spatial learning
2022-03-14 | PXD031136 | Pride
Transcriptomics Microarray analysis to identify genes that are differentially expressed in resting and in LPS-activated splenic B cells of Ebf1+/fl-RERTCre and Ebf1fl/fl-RERTCre mice
2012-04-02 | E-GEOD-36061 | biostudies-arrayexpress
Transcriptomics PDK1 is required for maintenance of CD4+ FoxP3+ regulatory T cell function [heterozygous]
2021-02-11 | GSE163659 | GEO
Transcriptomics Gene expression analysis of fetal liver pro-B cells upon deletion of Early B-cell Factor 1 (Ebf1)
2010-07-29 | E-GEOD-21454 | biostudies-arrayexpress
Transcriptomics PDK1 is required for maintenance of CD4+ FoxP3+ regulatory T cell function [Hemizygous]
2021-02-11 | GSE163662 | GEO
Genomics RNA seq of vhlfl/fl cd4 cre, hif1afl/fl vhlfl/fl cd4 cre and WT CD4 T cells before and after anti-CD3/CD28 stimulation
| PRJNA788478 | ENA
Transcriptomics Liver Homeostasis, Hepatocyte Ploidy, and Circadian Rhythm-controlled Gene Expression are Perturbed in Liver-Specific METTL3 Knockout Mice
2021-09-15 | GSE176113 | GEO