Interaction Partners of the Yeast Protein Disulfide Isomerase Eug1
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ABSTRACT: Results are from protein identification experiments where yeast expressing a FLAG-tagged form of Eug1 were treated with the thiol-reactive protein cross-linker DVSF. Eug1 was immunoprecipitated from cell lysates, and associated proteins were resolved by SDS-PAGE and extracted from the gel following staining with Coomassie blue. Subsequently, proteins in gel pieces were digested into peptides with trypsin, and peptides and their parent proteins were identified using LC-MS/MS. Biological replicate 1 of this experiment encompasses files MSB42821A through MSB42824A, and biological replicate 2 encompasses files MSB43174A through MSB43177A.
INSTRUMENT(S): ThermoFisher Fusion Lumos mass spectrometer
ORGANISM(S): Saccharomyces Cerevisiae
SUBMITTER: James West
PROVIDER: MSV000088258 | MassIVE | Sat Oct 23 09:18:00 BST 2021
REPOSITORIES: MassIVE
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