Proteomic and single-cell transcriptomic dissection of human plasmacytoid dendritic cell response to influenza virus

ABSTRACT: Plasmacytoid dendritic cells [pDCs] represent a rare innate immune subset uniquely endowed with the capacity to produce substantial amounts of type-I interferons [IFN-I]. This function of pDCs is critical for effective antiviral defenses and has been implicated in autoimmunity. While IFN-I and select cytokines have been recognized as pDC secreted products, a comprehensive agnostic profiling of the pDC secretome in response to a physiologic stimulus has not been reported. We applied LC-MS/MS to catalogue the repertoire of proteins secreted by pDCs in response to challenge with live influenza H1N1. Additionally, using single-cell RNA-seq [scRNA-seq], we perform multidimensional analyses of pDC transcriptional diversification following stimulation. Our data reveal an abundance of protein species released by pDCs in addition to IFN-I, and evidence highly specialized roles within the pDC population ranging from dedicated cytokine super-producers to cells with APC-like functions. Moreover, dynamic expression of transcription factors and surface markers characterize activated pDC fates.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Human Plasmacytoid Dendritic Cell

SUBMITTER: Peter Gregersen  

PROVIDER: MSV000088375 | MassIVE | Fri Nov 12 12:32:00 GMT 2021

REPOSITORIES: MassIVE