Dataset Information

Secretome Comparison of Mesenchymal Stromal Cells and Immature Osteoblasts

ABSTRACT: Osteoblasts are a key component of the endosteal hematopoietic stem cell (HSC) niche and have long been recognized with strong hematopoietic supporting activity. Osteoblast conditioned media (OCM) enhances the growth of hematopoietic progenitors in culture and modulate their engraftment activity. We aimed to characterize the hematopoietic supporting activity of OCM by comparing the secretome of immature osteoblasts to that of their precursor, mesenchymal stromal cells (MSC). Over 300 secreted proteins were quantified by mass spectroscopy in media conditioned with MSC or osteoblasts, with 47 being differentially expressed.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Nicolas Pineault

PROVIDER: MSV000088384 | MassIVE | Mon Nov 15 11:02:00 GMT 2021

SECONDARY ACCESSION(S): PXD017372

REPOSITORIES: MassIVE

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Insights Into the Hematopoietic Regulatory Activities of Osteoblast by Secretomics.

Hovey Owen O Pasha Roya R Lehmann Zoe Z Pineault Nicolas N

Proteomics 20200801 15-16

Osteoblasts are a key component of the endosteal hematopoietic stem cell niche and are recognized with strong hematopoietic supporting activity. Similarly, mesenchymal stromal cells (MSC)-derived osteoblast (M-OST) conditioned media (OCM) enhance the growth of hematopoietic progenitors in culture and modulate their engraftment activity. This article aims to characterize the hematopoietic supporting activity of OCM by comparing the secretome of M-OST to that of their precursor. Over 300 proteins ...[more]

PMID: 32666692

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