Proteomics

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Mapping the spatial proteome of metastatic cells in colorectal cancer

ABSTRACT: Here, we aimed to study metastasis mechanisms using spatial proteomics applied to the KM12 cell model of metastasis. KM12 cells were metabolically labelled using SILAC. SILAC has been successfully used for the analysis of proteome turnover and for the identification of changes in proteome localization as a consequence of DNA damage.10,23-24 Subcellular fractionation of the KM12 cells into five subcellular fractions corresponding to cytoplasm (CEB), plasma, mitochondria and ER/golgi membranes (MEB), nuclear (NEB), chromatin-bound (NEB-CBP) and cytoskeletal proteins (PEB) contributed to clarify the molecular mechanisms underlying CRC metastasis. Protein abundance and localization were measured in parallel for the five separate subcellular fractions, providing a map draft of the spatially deregulated protein complexes and networks in CRC metastasis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ignacio Casal  

PROVIDER: MSV000088431 | MassIVE | Tue Nov 23 01:05:00 GMT 2021

SECONDARY ACCESSION(S): PXD006656

REPOSITORIES: MassIVE

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Mapping the Spatial Proteome of Metastatic Cells in Colorectal Cancer.

Mendes Marta M   Peláez-García Alberto A   López-Lucendo María M   Bartolomé Rubén A RA   Calviño Eva E   Barderas Rodrigo R   Casal J Ignacio JI  

Proteomics 20171001 19

Colorectal cancer (CRC) is the second deadliest cancer worldwide. Here, we aimed to study metastasis mechanisms using spatial proteomics in the KM12 cell model. Cells were SILAC-labeled and fractionated into five subcellular fractions corresponding to: cytoplasm, plasma, mitochondria and ER/golgi membranes, nuclear, chromatin-bound and cytoskeletal proteins and analyzed with high resolution mass spectrometry. We provide localization data of 4863 quantified proteins in the different subcellular f  ...[more]

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