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Temporal dynamics of hippocampal protein expression in a familial model of Alzheimer's Disease

ABSTRACT: Mouse models of Alzheimer's Disease (AD) like 5XFAD, which show progression through various AD stages reflective of human pathology, are well-established tools for uncovering novel AD-related pathways. In addition, they permit temporal examination of the intermingling of AD-related pathways and can be used to potentially dissect initiating and propagating events in AD, which are critical for developing biomarkers or designing interventions in the early stages of the disease. The present research offers a targeted MS examination of five peptides of interest in a familial AD mice model with a design including variables of: tissue (hippocampus and tissue), time (three, six, and nine months), genetic background (5XFAD vs. WT), and sex (equal males and females). Parallel reaction monitoring (PRM) method examined five peptides, that were selected for monitoring four proteins (amyloid-beta A4, glial fibrillary acidic protein, clusterin, and beta-synuclein).

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Mark R. Chance

PROVIDER: MSV000088507 | MassIVE | Fri Dec 03 15:25:00 GMT 2021

REPOSITORIES: MassIVE

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Temporal dynamics of hippocampal protein expression in a familial model of Alzheimer's Disease

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