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Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality

ABSTRACT: There are no described quality assurance mechanisms for newly formed stem cells. We observed intimate interactions between macrophages and blood stem cells in zebrafish embryos. Stressed stem cells were marked by surface Calreticulin, which stimulates macrophage interaction as an eat me signal. Macrophage-stem cell interactions either lead to removal of cytoplasmic material and stem cell proliferation or resulted in complete stem cell engulfment. Calreticulin knock down or embryonic macrophage depletion reduced the number of stem cell clones into adulthood. Our work supports a model in which embryonic macrophages determine hematopoietic clonality by monitoring stem cell quality.

INSTRUMENT(S): HFX Orbitrap

ORGANISM(S): Danio Rerio (ncbitaxon:7955)

SUBMITTER: Leonard Zon

PROVIDER: MSV000088780 | MassIVE | Fri Feb 04 04:38:00 GMT 2022

SECONDARY ACCESSION(S): PXD031434

REPOSITORIES: MassIVE

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