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Label-free proteome analysis of small extracellular vesicles (sEV) expressing different HRS mutants.

ABSTRACT: Label-free proteome analysis of small extracellular vesicles (sEV) derived from WM9 cells expressing different HRS mutants: wild type (HRSWT), phospho-deficient (HRSS345A), and phospho-mimetic mutant HRS (HRSS345D).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Wei guo David W. Speicher

PROVIDER: MSV000088846 | MassIVE | Wed Feb 16 06:31:00 GMT 2022

SECONDARY ACCESSION(S): PXD031715

REPOSITORIES: MassIVE

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HRS phosphorylation drives immunosuppressive exosome secretion and restricts CD8+ T-cell infiltration into tumors.

Guan Lei L Wu Bin B Li Ting T Beer Lynn A LA Sharma Gaurav G Li Mingyue M Lee Chin Nien CN Liu Shujing S Yang Changsong C Huang Lili L Frederick Dennie T DT Boland Genevieve M GM Shao Guangcan G Svitkina Tatyana M TM Cai Kathy Q KQ Chen Fangping F Dong Meng-Qiu MQ Mills Gordon B GB Schuchter Lynn M LM Karakousis Giorgos C GC Mitchell Tara C TC Flaherty Keith T KT Speicher David W DW Chen Youhai H YH Herlyn Meenhard M Amaravadi Ravi K RK Xu Xiaowei X Guo Wei W

Nature communications 20220714 1

The lack of tumor infiltration by CD8+ T cells is associated with poor patient response to anti-PD-1 therapy. Understanding how tumor infiltration is regulated is key to improving treatment efficacy. Here, we report that phosphorylation of HRS, a pivotal component of the ESCRT complex involved in exosome biogenesis, restricts tumor infiltration of cytolytic CD8+ T cells. Following ERK-mediated phosphorylation, HRS interacts with and mediates the selective loading of PD-L1 t ...[more]

PMID: 35835783

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