Dataset Information

Comparative proteomic profiling of ectosomes derived from thyroid carcinoma and normal thyroid cells uncovers multiple proteins with functional implications in cancer

ABSTRACT: Proteins carried by tumor-derived ectosomes play an important role in cancer progression and are considered promising diagnostic markers. In the present study a shotgun nanoLC-MS/MS proteomic approach was applied to profile and compare the protein content of ectosomes released in vitro by normal human thyroid follicular epithelial Nthy-ori 3-1 cells and human anaplastic thyroid carcinoma (TC) 8305C cells. Additionally, pro-migratory and pro-proliferative effect of Nthy-ori 3-1- and 8305C-derived ectosomes exerted on the recipient cells was assessed in wound closure and Alamar Blue assays. A total of 919 proteins were identified in all replicates of 8305C-derived ectosomes, while Nthy-ori 3-1-derived ectosomes contained a significantly lower number of 420 identified proteins. Qualitative analysis revealed 568 proteins present uniquely in 8305C-derived ectosomes, suggesting their applicability in TC diagnosis and management. In addition, 8305C-derived ectosomes were able to increase proliferation and motility rate of the recipient cells, likely due to the ectosomal transfer of the identified cancer-promoting molecules. Description of ectosome protein content and its related functions provided the first insight on the role of ectosomes in TC development and progression. The results also indicated the applicability of some of these ectosomal proteins for further investigation regarding their potential as circulating TC biomarkers.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Malgorzata Przybylo

PROVIDER: MSV000088850 | MassIVE | Thu Feb 17 00:19:00 GMT 2022

SECONDARY ACCESSION(S): PXD031723

REPOSITORIES: MassIVE

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Comparative Proteomic Profiling of Ectosomes Derived from Thyroid Carcinoma and Normal Thyroid Cells Uncovers Multiple Proteins with Functional Implications in Cancer.

Surman Magdalena M Kędracka-Krok Sylwia S Wilczak Magdalena M Rybczyński Piotr P Jankowska Urszula U Przybyło Małgorzata M

Cells 20220331 7

Proteins carried by tumor-derived ectosomes play an important role in cancer progression, and are considered promising diagnostic markers. In the present study, a shotgun nanoLC-MS/MS proteomic approach was applied to profile and compare the protein content of ectosomes released in vitro by normal human thyroid follicular epithelial Nthy-ori 3-1 cells and human anaplastic thyroid carcinoma (TC) 8305C cells. Additionally, the pro-migratory and pro-proliferative effects of Nthy-ori 3-1- and 8305C- ...[more]

PMID: 35406748

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