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Nitrogen competition and proteome allocation in a surface-ocean microbial community

ABSTRACT: Dataset from a shipboard incubation experiment of an ocean surface-water microbial community sampled at 25m depth at Station ALOHA in the North Pacific Subtropical Gyre. Incubations were amended with ammonium, glutamate, leucine, nitrate and urea, in two isotopic variants: 15N (to track incorporation by various community members) and 14N (for quantitation of abundance changes by diDO-IPTL).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Microbial Community From North Pacific Ocean Surface Water

SUBMITTER: Jacob Waldbauer

PROVIDER: MSV000089118 | MassIVE | Wed Mar 23 19:16:00 GMT 2022

REPOSITORIES: MassIVE

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Publications

Tracking nitrogen allocation to proteome biosynthesis in a marine microbial community.

Zimmerman Amy E AE Podowski Justin C JC Gallagher Gwendolyn E GE Coleman Maureen L ML Waldbauer Jacob R JR

Nature microbiology 20230112 3

Microbial growth in many environments is limited by nitrogen availability, yet there is limited understanding of how complex communities compete for and allocate this resource. Here we develop a broadly applicable approach to track biosynthetic incorporation of <sup>15</sup>N-labelled nitrogen substrates into microbial community proteomes, enabling quantification of protein turnover and N allocation to specific cellular functions in individual taxa. Application to oligotrophic ocean surface wate ...[more]

PMID: 36635571

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