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DSSO Crosslinking of Partially Purified IFT-A from Tetrahymena thermophila

ABSTRACT: DSSO crosslinking data from two Tetrahymena thermophila samples: IEX fractions from chromatography of a cilia extract, and IFT-A-enriched fractions from preparative SEC of cilia extract.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Tetrahymena Thermophila (ncbitaxon:5911)

SUBMITTER: Edward Marcotte

PROVIDER: MSV000089131 | MassIVE | Fri Mar 25 11:03:00 GMT 2022

SECONDARY ACCESSION(S): PXD032818

REPOSITORIES: MassIVE

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Publications

Does AlphaFold2 model proteins' intracellular conformations? An experimental test using cross-linking mass spectrometry of endogenous ciliary proteins.

McCafferty Caitlyn L CL Pennington Erin L EL Papoulas Ophelia O Taylor David W DW Marcotte Edward M EM

Communications biology 20230415 1

A major goal in structural biology is to understand protein assemblies in their biologically relevant states. Here, we investigate whether AlphaFold2 structure predictions match native protein conformations. We chemically cross-linked proteins in situ within intact Tetrahymena thermophila cilia and native ciliary extracts, identifying 1,225 intramolecular cross-links within the 100 best-sampled proteins, providing a benchmark of distance restraints obeyed by proteins in their native assemblies. ...[more]

PMID: 37061613

Publication: 1/2

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