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Laser Capture Microdissection of Normal Kidney Biopsy Glomeruli and Tubulointerstitium Characterized by LC-MS/MS

ABSTRACT: The Kidney Precision Medicine Project (KPMP) is funded by the NIDDK for "the purpose of understanding and finding new ways to treat chronic kidney disease and acute kidney injury. " To accomplish this goal, the KPMP chose several technologies to successfully deliver their goals. One of the technologies chosen was using a combination of laser capture microdissection (LCM) in combination with LC -MS /MS to characterize quantitative protein changes in the diseased kidney. The data presented here is from the initial pilot study using normal kidney biopsies for proof of principal. Samples analyzed were either LCM collected glomeruli or tubulointerstitium. Protein was retrieved from the LCM collected tissue, proteins were trypsinized and samples run on the fusion orbitrap.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Samir Parikh

PROVIDER: MSV000089251 | MassIVE | Wed Apr 13 09:05:00 BST 2022

SECONDARY ACCESSION(S): PXD033207

REPOSITORIES: MassIVE

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