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Multimodal measurement of the transcriptome and proteome in single cells using nanoSPLITS

ABSTRACT: Single-cell and pooled cell proteomic data from alveolar type II epithelial cell line (C10) and axillary lymph node cell line (SVEC). Cells were sorted using cellenONE onto several nanoSPLITS chips. Cell lysates were then split for parallel RNAseq and proteomic analysis. For the proteomic sample prep all steps utilized the nanoPOTS sample handling robot. Lysates were reduced, treated with IAA, digested overnight with trypsin/Lys-C at 37C, and vacuum dried / stored at -20C. LC-MS/MS data was acquired using TIFF DDA methodology on a Thermo Tribrid mass spectrometer. Data was searched using FragPipe version 17.1 before downstream analysis in R.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Mus Musculus

SUBMITTER: Ying Zhu

PROVIDER: MSV000089280 | MassIVE | Tue Apr 19 09:53:00 BST 2022

REPOSITORIES: MassIVE

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