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Functional partitioning of transcriptional regulators by patterned charge blocks

ABSTRACT: GFP-MED1-IDR forms condensates when added to a soluble nuclear extract. Centrifugation fractionates the extract into a pellet fraction containing the proteins partitioned into MED1-IDR condensates and supernatant fraction containing proteins excluded from the MED1-IDR condensates. Two independent supernatant (LUM1_854558, LUM1_854560) and pellet (LUM1_854559, LUM1_854561) fractions were collected and subject to proteomic analysis to identify protein enriched in MED1-IDR condensates.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Benjamin Sabari

PROVIDER: MSV000089301 | MassIVE | Fri Apr 22 12:18:00 BST 2022

REPOSITORIES: MassIVE

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Functional partitioning of transcriptional regulators by patterned charge blocks

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