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Identification of the effect of a CRISPR interference knockdown of Borrelia burgdorferi strain B31 BB0838, a outer membrane lipoprotein flippase candidate, on surface lipoprotein secretion using Multidimensional Protein Identification Technology.

ABSTRACT: To identify the effect of a CRISPR interference knockdown of Borrelia burgdorferi strain B31 BB0838, a outer membrane lipoprotein flippase candidate, on surface lipoprotein secretion using Multidimensional Protein Identification Technology.

INSTRUMENT(S): LTQ (Thermo Scientific instrument model)

ORGANISM(S): Borrelia Burgdorferi B31 (ncbitaxon:224326)

SUBMITTER: Laurence Florens

PROVIDER: MSV000089990 | MassIVE | Wed Jul 27 14:47:00 BST 2022

SECONDARY ACCESSION(S): PXD035604

REPOSITORIES: MassIVE

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Publications

A Borrelia burgdorferi LptD homolog is required for flipping of surface lipoproteins through the spirochetal outer membrane.

He Huan H Pramanik Ankita S AS Swanson Selene K SK Johnson David K DK Florens Laurence L Zückert Wolfram R WR

Molecular microbiology 20230512 6

Borrelia spirochetes are unique among diderm bacteria in their lack of lipopolysaccharide (LPS) in the outer membrane (OM) and their abundance of surface-exposed lipoproteins with major roles in transmission, virulence, and pathogenesis. Despite their importance, little is known about how surface lipoproteins are translocated through the periplasm and the OM. Here, we characterized Borrelia burgdorferi BB0838, a distant homolog of the OM LPS assembly protein LptD. Using a CRISPR interference app ...[more]

PMID: 37170643

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