Proteomics

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Pull-Down of UCHL1 from living cells with small molecule probes GK13S, GK13R, and GK16S (1 uM)

ABSTRACT: UCHL1 was enriched from HEK293 cells (Homo Sapiens) using a probe (PullDown via NeutrAvidin beads). Protein enrichment as a function of substance addition was studied in comparison to a DMSO control.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Petra Janning  

PROVIDER: MSV000090045 | MassIVE | Mon Aug 01 01:52:00 BST 2022

SECONDARY ACCESSION(S): PXD035651

REPOSITORIES: MassIVE

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Publications

Structural basis for specific inhibition of the deubiquitinase UCHL1.

Grethe Christian C   Schmidt Mirko M   Kipka Gian-Marvin GM   O'Dea Rachel R   Gallant Kai K   Janning Petra P   Gersch Malte M  

Nature communications 20221010 1

Ubiquitination regulates protein homeostasis and is tightly controlled by deubiquitinases (DUBs). Loss of the DUB UCHL1 leads to neurodegeneration, and its dysregulation promotes cancer metastasis and invasiveness. Small molecule probes for UCHL1 and DUBs in general could help investigate their function, yet specific inhibitors and structural information are rare. Here we report the potent and non-toxic chemogenomic pair of activity-based probes GK13S and GK16S for UCHL1. Biochemical characteriz  ...[more]

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