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The proteome landscape of genome-wide genetic perturbations

ABSTRACT: Despite the massive research efforts of the post-genomics era, a significant fraction of genes remain without functional annotation. Proteomics holds the promise to close significant gaps, but has only recently reached the scalability and precision of genome-scale studies. Here we combine large-scale proteomics with functional genomics and use a streamlined platform for fast yeast proteomics to record quantitative proteomes of ~4,500 single knock-out strains of a genome-scale yeast knock-out collection.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Prof. Dr. Markus Ralser

PROVIDER: MSV000090136 | MassIVE | Mon Aug 15 11:06:00 BST 2022

SECONDARY ACCESSION(S): PXD036062

REPOSITORIES: MassIVE

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The proteomic landscape of genome-wide genetic perturbations.

Messner Christoph B CB Demichev Vadim V Muenzner Julia J Aulakh Simran K SK Barthel Natalie N Röhl Annika A Herrera-Domínguez Lucía L Egger Anna-Sophia AS Kamrad Stephan S Hou Jing J Tan Guihong G Lemke Oliver O Calvani Enrica E Szyrwiel Lukasz L Mülleder Michael M Lilley Kathryn S KS Boone Charles C Kustatscher Georg G Ralser Markus M

Cell 20230419 9

Functional genomic strategies have become fundamental for annotating gene function and regulatory networks. Here, we combined functional genomics with proteomics by quantifying protein abundances in a genome-scale knockout library in Saccharomyces cerevisiae, using data-independent acquisition mass spectrometry. We find that global protein expression is driven by a complex interplay of (1) general biological properties, including translation rate, protein turnover, the formation of protein compl ...[more]

PMID: 37080200

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