Proteomics

Dataset Information

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Aprosoff_SLX4_APMS_P131_VS12_6600


ABSTRACT: This dataset consists of 15 raw MS files and associated peak lists and results files, acquired on AB Sciex TripleTOF 6600 mass spectrometer operated in Data Dependent Acquisition mode. Samples were generated by Camila Aprosoff. FLAG-AP and Mass spectrometry acquisition was performed by Zhen-Yuan Lin. Analysis was performed by Cassandra Wong and Boris Dyakov. The files are associated with a manuscript submitted for publication by Camila Aprosoff et al. The main goal of this paper was to investigate SLX4 as a scaffold for DNA repair and chromatin-associated proteins. This dataset comprehensively maps the interactome of SLX4 using proximity labeling and affinity purification. Anne-Claude Gingras is the corresponding author of the manuscript (gingras@lunenfeld.ca); Anne-Claude Gingras should be contacted for questions on this dataset (gingras@lunenfeld.ca) This submission is associated with 3 Supplementary Files (in addition to this README file) Table 1 describes the composition of this dataset Table 2 lists all the peptide identification evidence (as per iProphet) Table 3 lists the SAINTexpress interactions

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras  

PROVIDER: MSV000090314 | MassIVE |

SECONDARY ACCESSION(S): PXD036690

REPOSITORIES: MassIVE

Dataset's files

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Publications

Comprehensive Interactome Mapping of the DNA Repair Scaffold SLX4 Using Proximity Labeling and Affinity Purification.

Aprosoff Camila M CM   Dyakov Boris J A BJA   Cheung Vivian H W VHW   Wong Cassandra J CJ   Palandra Mikaela M   Gingras Anne-Claude AC   Wyatt Haley D M HDM  

Journal of proteome research 20230418 6


The DNA repair scaffold SLX4 has pivotal roles in cellular processes that maintain genome stability, most notably homologous recombination. Germline mutations in <i>SLX4</i> are associated with Fanconi anemia, a disease characterized by chromosome instability and cancer susceptibility. The role of mammalian SLX4 in homologous recombination depends critically on binding and activating structure-selective endonucleases, namely SLX1, MUS81-EME1, and XPF-ERCC1. Increasing evidence indicates that cel  ...[more]

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