Proteomics

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Transfer of the high-quality sugar metabolic model of Aspergillus niger is reliable within the same fungal phylum, but limited for fungi of other phyla


ABSTRACT: Proteomic data from fungi; intracellular-proteome of five of the studied fungi (A. niger, A. nidulans, P. subrubescens, T. reesei and P. chrysosporium) grown on nine different monosaccharide conditions (L-arabinose, D-fructose, D-galacturonicAcid, D-galactose, D-glucuronicAcid, D-glucose, D-mannose, L-rhamnose, D-xylose) was analyzed to reveal the fungal response to each specific sugar at the proteome level ; 3 biological replicates. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline against FASTA files obtained from Mycocosm.

INSTRUMENT(S): Thermo Scientific Q-Exactive Plus

ORGANISM(S): Aspergillus Niger, Aspergillus Nidulans, Penicillium Subrubescens, Trichoderma Reesei, Phanerochaete Chrysosporium

SUBMITTER: Ronald de Vries  

PROVIDER: MSV000090477 | MassIVE | Wed Oct 05 15:29:00 BST 2022

SECONDARY ACCESSION(S): PXD037196

REPOSITORIES: MassIVE

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Fungi play a critical role in the global carbon cycle by degrading plant polysaccharides to small sugars and metabolizing them as carbon and energy sources. We mapped the well-established sugar metabolic network of <i>Aspergillus niger</i> to five taxonomically distant species (<i>Aspergillus nidulans</i>, <i>Penicillium subrubescens</i>, <i>Trichoderma reesei</i>, <i>Phanerochaete chrysosporium</i> and <i>Dichomitus squalens</i>) using an orthology-based approach. The diversity of sugar metabol  ...[more]

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