Proteomics

Dataset Information

0

Comparative proteomics of Chlamydia muridarum strains in the absence of the conserved tail specific protease


ABSTRACT: The role of chlamydial proteases in the pathogenesis of Chlamydia spp. has remained largely unknown. In the present study we have generated a Chlamydia muridarum strain that is a null mutant of tail specific protease (TSP). TSP has conserved orthologs in other chlamydial species and structurally similar homologs in multiple free-living and intracellular bacteria. Similar to the roles of its homologs, TSP seems to play a crucial role in chlamydial resistance to elevated temperatures. Importantly, the tsp null mutant is also heavily attenuated in the mouse urogenital tract, indicating that TSP has a crucial role in chlamydial pathogenicity. We have utilized quantitative proteomics to identify chlamydial proteins that have altered abundances in the tsp null mutant compared to wild-type and an isogenic recombinant of the mutant. These proteins indicate potential substrates of this protease and hints toward the molecular function of TSP.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090) Chlamydia Muridarum (ncbitaxon:83560)

SUBMITTER: David E. Nelson   Amber L. Mosley  

PROVIDER: MSV000090509 | MassIVE | Mon Oct 10 12:50:00 BST 2022

SECONDARY ACCESSION(S): PXD037282

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-11-21 | E-GEOD-41844 | biostudies-arrayexpress
2013-11-21 | GSE41844 | GEO
2019-04-09 | PXD002186 | Pride
2014-12-12 | GSE64065 | GEO
2014-12-12 | E-GEOD-64065 | biostudies-arrayexpress
2021-08-11 | GSE171959 | GEO
2012-09-18 | E-GEOD-37747 | biostudies-arrayexpress
2023-02-15 | GSE224117 | GEO
2012-09-18 | GSE37747 | GEO
2009-08-01 | E-GEOD-12454 | biostudies-arrayexpress