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Simultaneous Screening and Quantitation of Human Milk Oligosaccharides by Liquid Chromatography – Mass Spectrometry

ABSTRACT: An analytical method which enables the simultaneous quantitation of seven abundant HMOs (i.e., 2-FL, LNT, LNFP I, LNDFH I, 3-SL, DSLNT, and 6-SL) and the simultaneous screening of a wide variety of HMOs is presented. First, sample preparation included an SPE purification step that enabled a quantitative extraction of HMOs. Then, isolated HMOs are analyzed using hydrophilic interaction liquid chromatography (HILIC) coupled to a Q-Exactive Plus Mass Spectrometer from Thermofisher operating in polarity switching mode with a total runtime of 32.5 minutes and with help of an inclusion list constructed from the information contained in the NIST Milk Oligosaccharide MS Library. Three complementary data processing strategies were employed: (1) HMO quantitation, (2) screening, and (3) molecular networking.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Isabel Ten-Domenech

PROVIDER: MSV000090546 | MassIVE | Tue Oct 18 01:03:00 BST 2022

REPOSITORIES: MassIVE

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