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The detection of plasma proteins Rainbow trout adhered to the surface of Aeromonas hydrophila

ABSTRACT: The effect of rainbow trout plasma on the conditionally pathogenic gram-negative bacterium A. hydrophila, the causative agent of septicemia, has been studied. It has been shown that the native blood plasma of conditionally healthy cultured trout does not have an antimicrobial effect on bacteria. However, the high-molecular fraction of plasma of trout conditionally immunized with F. psychrophilum had an antimicrobial effect on A. hydrophila. It has been shown that the plasma of supposedly immunized trout prevents the reproduction of bacteria and disrupts their morphology. Using HPLC-MS/MS, we investigated potentially immune plasma proteins that adhere to the surface of bacteria. Proteins that remained on the surface of bacteria after a series of washings were studied. The results of the research may be useful for studying immune proteins of teleost fish and other vertebrates recognizing PAMPs of Gram-negative bacteria and for the cross-resistance of trout between phylogenetically unrelated bacterial species.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Aeromonas Hydrophila (ncbitaxon:644) Oncorhynchus Mykiss (ncbitaxon:8022)

SUBMITTER: Irina V. Sukhovskaya

PROVIDER: MSV000090605 | MassIVE | Thu Oct 27 23:39:00 BST 2022

SECONDARY ACCESSION(S): PXD037789

REPOSITORIES: MassIVE

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