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Cilia-APEX proteomics profiling of Arl6-/- vs wiltype reveal UbK63 retrieval adaptors

ABSTRACT: Ascrobate peroxidase (APEX) was expressed as a fusion protein and directed to primary cilia of mouse inner medullary collecting duct (mIMCD-3) cells, where it was used for proximity labeling to biotinylate proteins, which are subsequently enriched by streptavidin chromatography and identified by mass spectrometry. Cilia-APEX proteomics profiling compared the cilia proteomes of wild-type and Arl6-/- cilia (generated by CRISPR/Cas9-mediated genome editing) by spectral counting.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Maxence V. Nachury

PROVIDER: MSV000090633 | MassIVE | Wed Nov 02 09:39:00 GMT 2022

SECONDARY ACCESSION(S): PXD037886

REPOSITORIES: MassIVE

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