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DIA_staggered_LFQ_Benchmark_High-Performance_QE-HF_30,90,150minGradient

ABSTRACT: LFQ Benchmark with samples from commercial digests according to Kuharev, Joerg, et al (2015). Sample A: 5% E.coli, 30 % Yeast, 65% Human, 0.9ug on column. Sample B: 20% E.coli, 15% Yeast, 65% Human, 0.9ug on column. (4 replicates per gradient length, one aliquot per 4 samples from one mastermix per sample type). Expected log2 fold-changes of -2,0,+1 for E.coli, Human, Yeast. Sample C: 0.9ug on column, average of A and B used to build enhanced spectral library by Gas-Phase-Fractionation. Sample C files are here for completeness, obtained libraries can lead to improvements but I do not use refined libraries for my applications anymore, use at own caution and risk. Notably, the chromatography was performed with uPAC analytical column, resulting in sharp and symmetric <20s short chromatograms. Gradients (30,90,150 min) were 2-sloped and linear, with the last third of the gradient encompassing half of the %B increase. Analysis with Spectronaut might not be ideal as MS1 was recorded at 30k resolution. This dataset, in particular the 90min gradient ones, can possibly lead to the strongest LFQ benchmark results and might serve as upper standards in comparisons.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Andrej Shevchenko

PROVIDER: MSV000090837 | MassIVE | Mon Dec 05 12:28:00 GMT 2022

REPOSITORIES: MassIVE

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