Shotgun proteomics of co-cultured cells from different species as an alternative approach to detect intercellular protein transfer. Part 2: OP9 cells

ABSTRACT: NOTE: In this project we used two types of cells: the human CCRF-CEM cells, and the OP9 cells from mouse. This dataset includes only the peak and raw data files of the OP9 cells harvested from monocultures and co-cultures. The files corresponding to the CCRF-CEM cells can be downloaded from the MassIVE Dataset MSV000090911. The files mqpar.txt, SearchEngineResults14sep22.zip and FastaUniProtdatabases.zip are the same for the CCRF-CEM and OP9 datasets. Project description Cellular interactions within the bone marrow microenvironment modulate the properties of subsets of leukemic cells leading to the development of drug-resistant phenotypes. The intercellular transfer of proteins and organelles contributes to this process but the set of transferred proteins and their effects in the receiving cells remain unclear. This study aimed to detect the intercellular protein transfer from mouse bone marrow stromal cells (OP9 cell line) to human T-lymphoblasts (CCRF-CEM cell line) using nanoLC-MS/MS-based shotgun proteomics in a 3D co-culture system. After 24 hours of co-culture, 1513 and 67 proteins from human and mouse origin respectively, were identified in human CCRF-CEM cells. The presence of mouse proteins in the human cell line, detected by analyzing the differences in amino acid sequences of orthologous peptides, was interpreted as the result of intercellular transfer. Although further validation is necessary, our results suggest that shotgun proteomic analyses of co-cultured cells from different species could be a simple option to get a preliminary survey of the proteins exchanged among interacting cells.

INSTRUMENT(S): impact II

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Hector Quezada Pablo  

PROVIDER: MSV000090943 | MassIVE | Tue Dec 20 18:13:00 GMT 2022

SECONDARY ACCESSION(S): PXD038979

REPOSITORIES: MassIVE