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L-tryptophan and copper interactions linked to reduced colibactin genotoxicity in pks+ Escherichia coli

ABSTRACT: Untargeted metabolomics was performed on a time series of supernatants from E. coli ATCC 25922 cultures growing in either MM29 medium (PMID 35273143) or chopped meat glucose (CMG) medium (Anaerobe Systems, CA USA). Overnight E. coli ATCC 25922 cultures were diluted 1:100 into fresh medium (CMG or MM29) containing 100 uM of a ClbP activity-based probe (PMID 31059217) and culture supernatant samples were taken for metabolomics at six time points after culture inoculation: 0, 4, 8, 22, 25, 29h.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: Andrew Tolonen

PROVIDER: MSV000091164 | MassIVE | Thu Jan 26 14:29:00 GMT 2023

REPOSITORIES: MassIVE

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