Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Supplementary Dataset 11 - Alkyne-2PCA clicked to biotin-disulfide-azide enrichment with E coli proteome derived peptide libraries

ABSTRACT: Alkyne-2PCA clicked to biotin-disulfide-azide enrichment with E coli proteome derived peptide libraries

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: Amy Weeks

PROVIDER: MSV000091257 | MassIVE | Sat Feb 11 17:37:00 GMT 2023

SECONDARY ACCESSION(S): PXD040054

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Supplementary Dataset 10 - Alkyne-2PCA clicked to biotin-azide enrichment with E coli proteome-derived peptide libraries

Project description:Alkyne-2PCA clicked to biotin-azide enrichment with E coli proteome-derived peptide libraries

2023-02-11 | MSV000091255 | MassIVE

Supplementary Dataset 12 - Alkyne-2PCA clicked to biotin-DADPS-azide enrichment with E coli proteome derived peptide libraries

Project description:Alkyne-2PCA clicked to biotin-DADPS-azide enrichment with E coli proteome derived peptide libraries

2023-02-11 | MSV000091258 | MassIVE

Supplementary Dataset 14 - Alkyne-2PCA clicked to biotin-Diazo-azide enrichment with E coli proteome derived peptide libraries

Project description:Alkyne-2PCA clicked to biotin-Diazo-azide enrichment with E coli proteome derived peptide libraries

2023-02-11 | MSV000091260 | MassIVE

Supplementary Dataset 13 - Alkyne-2PCA clicked to biotin-Dde-azide enrichment with E coli proteome derived peptide libraries

Project description:Alkyne-2PCA clicked to biotin-Dde-azide enrichment with E coli proteome derived peptide libraries

2023-02-11 | MSV000091259 | MassIVE

Supplementary Dataset 7 - Human proteome-derived peptide library generated with trypsin; treated with 10 mM alkyne-2PCA for 20 h at 37C; clicked with biotin azide

Project description:Human proteome-derived peptide library generated with trypsin; treated with 10 mM alkyne-2PCA for 20 h at 37C; clicked with biotin azide

2023-02-11 | MSV000091252 | MassIVE

Cu-Catalyzed Azide-Alkyne-Thiol Reaction Forms Ubiquitous Background in Chemical Proteomics Studies

Project description:We report here a Cu-catalyzed azide-alkyne-thiol reaction forming thiotriazoles as the major by-product under widely used bioorthogonal protein labelling “click” conditions. The development of Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) had tremendous impact on many biological discoveries. However, the considered chemoselectivity of CuAAC is hampered by high reactivity of cysteine free thiols yielding thiotriazole protein conjugates. The reaction by-products generate false positive protein hits in “functional” proteomic studies. The reported detail investigation of conjugates between chemical probes containing terminal alkynes, azide-tags and cell lysates reveals formation of thiotriazoles, which can be readily detected by in-gel fluorescence scanning or after peptide and protein enrichment by MS-based proteomics. The produced fluorescent bands or enriched proteins may not result from the important enzymatically driven reaction and can be falsely assigned as hits. This study provides a complete list of the most common background proteins. The knowledge of this previously overlooked reactivity now leads to improved experimental design. Here, we present modified CuAAC conditions, which avoids the undesired product formation and diminishes the background.

2024-01-12 | PXD043402 | Pride

Supplementary Dataset 6 - Human proteome-derived peptide library generated with trypsin; treated with 10 mM alkyne-2PCA for 20 h at 37C

Project description:Human proteome-derived peptide library generated with trypsin; treated with 10 mM alkyne-2PCA for 20 h at 37C

2023-02-11 | MSV000091251 | MassIVE

Ecoli W3110_0.01 M sodium azide treatment vs untreated, timecourse

Project description:Responses of Escherichia coli W3110 after treatment with 0.01 M Sodium Azide in Bonner-Vogel medium + 50 ug/ml Tryptophan Keywords: time course

2006-03-08 | GSE4372 | GEO

A concomitant allylic azide rearrangement/intramolecular azide-alkyne cycloaddition sequence.

Project description:An intramolecular Huisgen cycloaddition of an interconverting set of isomeric allylic azides with alkynes affords substituted triazoles in high yield. The stereoisomeric vinyl-substituted triazoloxazines formed depend on the rate of cycloaddition of the different allylic azide precursors when the reaction is carried out under thermal conditions. In contrast, dimerized macrocyclic products were obtained when the reaction was done using copper(I)-catalyzed conditions, demonstrating the ability to control the reaction products through changing conditions.

| S-EPMC3993870 | biostudies-other

Alkyne-probe pull-down in LAPC4-CR cells

Project description:LAPC4-CR cells were treated with alkyne-probe or DMSO for 2 h in triplicate, lysed and conjugated with biotin-picolyl-azide. Biotinylated proteins were pulled down with neutravidin agarose. After the pulldown, the neutravidin agarose beads were treated with trypsin, and the tryptic peptides were labelled with TMT6 and analyzed by tandem mass spectrometry. DMSO = Reporter 126-128, alkyne-probe = Reporter 129-131. Two replicate injections.

2023-11-30 | MSV000093542 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data