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IBAQ Quantification of the Caulerpa prolifera Proteome

ABSTRACT: MaxQuant 2.1.3.0 iBAQ analysis of triplicate Caulerpa prolifera samples. Whole algae samples were frozen, ground, extracted with acetone and phenol, recovered by precipitation, and solubilized for SDS-PAGE. For each of the triplicate samples the gel lane was cut into 12 gel slices and prepared by in-gel trypsin digest for mass spectrometry on an Orbitrap Fusion Lumos mass spectrometer. Data were collected using an LC/MS-MS DDA method with a 60 min gradient and stepped HCD.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Caulerpa Prolifera (ncbitaxon:76314)

SUBMITTER: Edward Marcotte

PROVIDER: MSV000091436 | MassIVE | Wed Mar 08 09:44:00 GMT 2023

SECONDARY ACCESSION(S): PXD040688

REPOSITORIES: MassIVE

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