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SAD-1 kinase controls presynaptic phase separation by relieving SYD-2/Liprin-? autoinhibition

ABSTRACT: C. elegans SAD-1 kinase in vitro phosphorylation of SYD-2.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Kang Shen

PROVIDER: MSV000091471 | MassIVE | Fri Mar 10 13:16:00 GMT 2023

REPOSITORIES: MassIVE

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SAD-1 kinase controls presynaptic phase separation by relieving SYD-2/Liprin-α autoinhibition.

McDonald Nathan A NA Tao Li L Dong Meng-Qiu MQ Shen Kang K

bioRxiv : the preprint server for biology 20230612

Neuronal development orchestrates the formation of an enormous number of synapses that connect the nervous system. In developing presynapses, the core active zone structure has been found to assemble through a liquid-liquid phase separation. Here, we find that the phase separation of SYD-2/Liprin-α, a key active zone scaffold, is controlled by phosphorylation. Using phosphoproteomics, we identify the SAD-1 kinase to phosphorylate SYD-2 and a number of other substrates. Presynaptic assembly is im ...[more]

PMID: 37398223

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