Phaeodactylum tricornutum proteome from SEC and IEX fractionation of native extract
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ABSTRACT: Phaeodactylum tricornutum (UTEX 646) grown without silica were used to generate a native protein extract. Extraction involved multiple freeze/thaw cycles, sonication, and solubilization in buffer containing 1% NP40. Following chromatographic separation by SEC or IEX the fractions were reduced/alkylated and digested with trypsin for LC-MS/MS on an orbitrap fusion. Data were collected using a 75 minute top speed DDA method with CID.
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Phaeodactylum Tricornutum (ncbitaxon:2850)
SUBMITTER: Edward Marcotte
PROVIDER: MSV000091621 | MassIVE | Mon Apr 03 13:45:00 BST 2023
SECONDARY ACCESSION(S): PXD050670
REPOSITORIES: MassIVE
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