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Rotifer proteome from SEC fractionated Brachionus rotundiformis

ABSTRACT: Brachionus rotundiformis were obtained by filtration of a non-axenic culture to remove feeder algae. Rotifers were flash frozen, ground in liquid nitrogen, and lysed by resuspension in buffer containing 0.2% NP40 with dounce homogenization. Native protein lysate was fractionated on an HPLC SEC and all fractions reduced/alkylated and digested with trypsin for LC-MS/MS. Data collection was with a 75 minute top speed DDA method on an Orbitrap fusion using HCD. Data processing was with MSBlender.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Brachionus Rotundiformis (ncbitaxon:96890)

SUBMITTER: Edward Marcotte

PROVIDER: MSV000091679 | MassIVE | Tue Apr 11 10:53:00 BST 2023

SECONDARY ACCESSION(S): PXD050673

REPOSITORIES: MassIVE

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