Proteomics

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Protein Identification in Tetrahymena thermophila cilia IEX fractions used for XL-MS


ABSTRACT: Tetrahymena thermophila SB715 were deciliated by pH shock substituting HEPES for Tris in all subsequent buffers. Ciliry proteins were solubilized with buffer containing 1%NP40, and fractionated by HPLC on a mixed-bed IEX column. DSSO was added to 0.5 mM to all collected fractions. Crosslinking was quenched with Tris pH 8.0 at 28 mM and fractions were reduced, alkylated, and digested with trypsin for mass spectrometry. Spectra were collected on an Orbitrap Fusion Lumos using a DDA MS2-MS3 method for both protein and crosslink identification. Data in this deposition include the RAW files and MSBlender pipeline files used for protein identification. Crosslink analysis will be deposited separately.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Tetrahymena Thermophila (ncbitaxon:5911)

SUBMITTER: Edward Marcotte  

PROVIDER: MSV000091710 | MassIVE | Thu Apr 13 14:53:00 BST 2023

SECONDARY ACCESSION(S): PXD050674

REPOSITORIES: MassIVE

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