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Metabolism of plant stem cells under low oxygen: metabolic rewiring by phytoglobin underlying stem cell functionality

ABSTRACT: Root growth in maize (Zea mays L.) is regulated by the activity of the quiescent center (QC) stem cells located within the root apical meristem. Here we show that despite being highly hypoxic under normal oxygen tension, QC stem cells are vulnerable to hypoxic stress which causes their degradation with the subsequent inhibition of root growth. Under low oxygen, QC stem cells become depleted of starch and soluble sugars, and exhibit reliance on glycolytic fermentation with the impairment of the TCA cycle through the depressed activity of several enzymes including pyruvate dehydrogenase (PDH). Corn root sample pools from three genotypes were subjected to differential protein analysis using TMT10 isotopic labelling. Run S1 contained three biological replicates of each genotype under baseline condition, while run S2 contained three biological replicates of each genotype under a low oxygen stress condition. The final TMT channel in both runs contained a baseline pool across all genotypes to serve as a cross-run expression anchor. Each set was a 2D-LC-MS2 run with 10 fractions of 90 minutes each.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Zea Mays (ncbitaxon:4577)

SUBMITTER: Claudio Stasolla

PROVIDER: MSV000091770 | MassIVE | Fri Apr 21 10:09:00 BST 2023

REPOSITORIES: MassIVE

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Metabolism of plant stem cells under low oxygen tension: metabolic reprogramming by phytoglobin (Pgb1) underlying stem cell functionality

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