Proteomics

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Lactate and Immunomagnetic-purified iPSC-derived Cardiomyocytes Generate Comparable Engineered Cardiac Tissue Constructs

ABSTRACT: top down and bottom up proteomics files for Lactate and Immunomagnetic-purified iPSC-derived Cardiomyocytes Generate Comparable Engineered Cardiac Tissue Constructs

INSTRUMENT(S): timsTOF Pro, impact

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ying Ge  

PROVIDER: MSV000091869 | MassIVE | Fri May 05 15:47:00 BST 2023

REPOSITORIES: MassIVE

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Lactate- and immunomagnetic-purified hiPSC-derived cardiomyocytes generate comparable engineered cardiac tissue constructs.

Rossler Kalina J KJ   de Lange Willem J WJ   Mann Morgan W MW   Aballo Timothy J TJ   Melby Jake A JA   Zhang Jianhua J   Kim Gina G   Bayne Elizabeth F EF   Zhu Yanlong Y   Farrell Emily T ET   Kamp Timothy J TJ   Ralphe J Carter JC   Ge Ying Y  

JCI insight 20240109 1

Three-dimensional engineered cardiac tissue (ECT) using purified human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) has emerged as an appealing model system for the study of human cardiac biology and disease. A recent study reported widely used metabolic (lactate) purification of monolayer hiPSC-CM cultures results in an ischemic cardiomyopathy-like phenotype compared with magnetic antibody-based cell sorting (MACS) purification, complicating the interpretation of studies usi  ...[more]

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