Proteomics

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Label-free quantification of NMT1 and NMT2 knockout as well as PCLX-001 treated HAP1 cells

ABSTRACT: HAP1 cells with an NMT1, NMT2, or double knockout (as well as wildtype controls) were prepared by in-gel trypsin digestion and analyzed by LC-MS/MS label-free quantification (data-dependent acquisition). Additionally, HAP1 cells were treated with PCLX-001, an NMT inhibitor, at EC50 and EC90 (as well as untreated controls) and analyzed as described previously. (n=3)

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Olivier Julien  

PROVIDER: MSV000091913 | MassIVE | Wed May 10 22:18:00 BST 2023

REPOSITORIES: MassIVE

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Publications

Multiomics analysis identifies oxidative phosphorylation as a cancer vulnerability arising from myristoylation inhibition.

Beauchamp Erwan E   Gamma Jay M JM   Cromwell Christopher R CR   Moussa Eman W EW   Pain Rony R   Kostiuk Morris A MA   Acevedo-Morantes Claudia C   Iyer Aishwarya A   Yap Megan M   Vincent Krista M KM   Postovit Lynne M LM   Julien Olivier O   Hubbard Basil P BP   Mackey John R JR   Berthiaume Luc G LG  

Journal of translational medicine 20240507 1

<h4>Background</h4>In humans, two ubiquitously expressed N-myristoyltransferases, NMT1 and NMT2, catalyze myristate transfer to proteins to facilitate membrane targeting and signaling. We investigated the expression of NMTs in numerous cancers and found that NMT2 levels are dysregulated by epigenetic suppression, particularly so in hematologic malignancies. This suggests that pharmacological inhibition of the remaining NMT1 could allow for the selective killing of these cells, sparing normal cel  ...[more]

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