Dataset Information

A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant

ABSTRACT: Green leaf volatiles (GLVs) are short-chain oxylipins that are emitted from plants in response to stress. Previous studies have shown that oral secretions (OS) of the tobacco hornworm Manduca sexta, introduced into plant wounds during feeding, catalyze the re-arrangement of GLVs from Z-3- to E-2-isomers. This change in the volatile signal however is bittersweet for the insect as it can be used by their natural enemies, as prey location cue. Here we identified a (3Z):(2E)-hexenal isomerase (Hi-1) in M. sexta OS that catalyzes the conversion of the GLV Z-3-hexenal to E-2-hexenal. Hi-1 mutants that were raised on a GLV27 free diet showed developmental disorders, indicating that Hi-1 also metabolizes other substrates important for the insects development. Phylogenetic analysis placed Hi-1 within the GMCb-subfamily and showed that Hi-1 homologs from other lepidopterans could catalyze similar reactions. Our results indicate that Hi- 1 not only modulates the plants GLV-bouquet but also functions in insect development.

INSTRUMENT(S): Q-TOF (Micromass instrument model)

ORGANISM(S): Manduca Sexta (ncbitaxon:7130)

SUBMITTER: Gertjan Kramer

PROVIDER: MSV000092011 | MassIVE | Tue May 23 03:12:00 BST 2023

REPOSITORIES: MassIVE

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A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant.

Lin Yu-Hsien YH Silven Juliette J M JJM Wybouw Nicky N Fandino Richard A RA Dekker Henk L HL Vogel Heiko H Wu Yueh-Lung YL de Koster Chris C Große-Wilde Ewald E Haring Michel A MA Schuurink Robert C RC Allmann Silke S

Nature communications 20230628 1

Green leaf volatiles (GLVs) are short-chain oxylipins that are emitted from plants in response to stress. Previous studies have shown that oral secretions (OS) of the tobacco hornworm Manduca sexta, introduced into plant wounds during feeding, catalyze the re-arrangement of GLVs from Z-3- to E-2-isomers. This change in the volatile signal however is bittersweet for the insect as it can be used by their natural enemies, as a prey location cue. Here we show that (3Z):(2E)-hexenal isomerase (Hi-1) ...[more]

PMID: 37380635

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