Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Leaf samples pilot study Papua New Guinea

ABSTRACT: Pilot study on leaves from Papua New Guinea showing soil toxicity, extracted in RNAlater in one case and MQ water in another, also MQ blanks

INSTRUMENT(S): QE Orbitrap

ORGANISM(S): Unknown Leaves Png

SUBMITTER: Jeffrey Hawkes

PROVIDER: MSV000092034 | MassIVE | Thu May 25 08:53:00 BST 2023

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Identification of new full length Arabidopsis genes - pilot arrays

Project description:Identification of new and unpredicted full length Arabidopsis genes. Examination of cRNA prepared from Arabidopsis thaliana ecotype Columbia light grown 7-day old seedlings, light grown 7-day old seedlings treated at 4 degrees C for 24hrs, and etiolated 7-day old seedlings using pilot genome tiling arrays. Total RNA was isolated from seedlings with the TRIzol reagent procedure of Invitrogen, then poly(A)+ RNA was purified with Qiagen oligotex. One microgram poly(A)+ RNA was converted into ds-cDNA using T7-oligo(dT) primers. Biotin-labeled cRNA was generated by in vitro transcription reactions using ENZO labeling kit, fragmented and then 20 micrograms of fragmented cRNA were hybridized to the arrays according to Affymetrix instructions. Keywords: other

2003-10-30 | GSE604 | GEO

Mechanisms of response to silver nanoparticles Enchytraeus albidus (Oligochaeta): survival, reproduction and gene expression profile

Project description:Due to its antimicrobial activity, silver nanoparticles (Ag-NPs) are among the most used NPs worldwide, yet little information is available regarding their effects, particularly in soil dwelling organisms. Enchytraeids (Oligochaeta) are important members of the soil fauna which actively contribute to the acceleration of organic matter decomposition and nutrient recycling processes. Hence, for hazard and risk assessment it is important to provide toxicity data for these organisms and to understand more in regard to the mode of action of Ag-NPs within organism. To study this we conducted toxicity experiments using the OECD standard guideline, testing Ag-NPs and AgNO3, having assessed survival, reproduction and differential gene expression. Population toxicity responses were assessed showing higher toxicity for the AgNO3. In an attempt to understand the mode of action we performed transcription profiling using the microarray. Gene expression profile of Enchytraeus albidus was analysed after 2 days of exposure to 100 and 200 mg/kg of two silver forms (nanoparticles and salt_silver nitrate) in OECD soil. Three biological replicates per test treatment and control (clean OECD soil) were used.

2013-05-22 | E-GEOD-42431 | biostudies-arrayexpress

Salmonella compare pilot

Project description:Salmonella compare pilot

| PRJEB27347 | ENA

Salmonella compare pilot

Project description:Salmonella compare pilot

| PRJEB27350 | ENA

Chlamydia trachomatis pilot

Project description:Chlamydia trachomatis pilot

| PRJEB2214 | ENA

Mycobacterium tuberculosis pilot

Project description:Mycobacterium tuberculosis pilot

| PRJEB2221 | ENA

Illinois EBP Pilot

Project description:Illinois EBP Pilot

| PRJNA844590 | ENA

Gene expression profiling as a tool for sediment risk assessment: a pilot study using Caenorhabditis elegans whole genome microarrays

Project description:Toxicity of river sediments are assessed using whole sediment toxicity tests with benthic organisms. The challenge, however, is the differentiation between multiple effects caused by complex contaminant mixtures and the unspecific toxicity endpoints such as survival, growth or reproduction. Moreover, natural sediment properties, such as grain size distribution and organic carbon content, can influence the test parameters by masking pollutant toxicity. The use of gene expression profiling facilitates the identification of transcriptional changes at the molecular level that are specific to the bioavailable fraction of pollutants. The nematode Caenorhabditis elegans is ideally suited for these purposes, as (i) it can be exposed to whole sediments, and (ii) its genome is fully sequenced and widely annotated. In this pilot study we exposed C. elegans for 48 h to three sediments varying in degree of contamination with e.g. heavy metals and organic pollutants. Following the exposure period, gene expression was profiled using a whole genome DNA-microarray approach.

2008-06-25 | GSE11837 | GEO

Gene expression profiling as a tool for sediment risk assessment: a pilot study using Caenorhabditis elegans whole genome microarrays

2008-06-25 | E-GEOD-11837 | biostudies-arrayexpress

Geuvadis RNA-seq five individual pilot

Project description:Pilot RNA-seq dataset created by the Geuvadis consortium (www.geuvadis.org)

2010-04-14 | E-GEUV-4 | biostudies-arrayexpress