Database_Gliotoxin_transduction

ABSTRACT: A. fumigatus is a saprophytic fungus that can cause a variety of human diseases known as aspergillosis. Mycotoxin gliotoxin (GT) production is important for its virulence and has to be tightly regulated to avoid excess production and toxicity to the fungus. GT self-protection by GliT oxidoreductase and GtmA methyltransferase is related to the cellular sub-location of these enzymes and how GT can be sequestered from the cytoplasm to avoid increased cell damage. Here, we show that GliT:GFP and GtmA:GFP are localized in the cytoplasm and in vacuoles during GT production. Peroxisomes are also required for proper GT production and self-defense. The Mitogen-Activated kinase MpkA is essential for GT production and self-protection, interacts physically with GliT and GtmA and it is necessary for their regulation and subsequent presence in the vacuoles. Our work emphasizes the importance of dynamic compartmentalization of cellular events for the GT production and self-defense.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Aspergillus Fumigatus (ncbitaxon:746128)

SUBMITTER: Taicia Pacheco Fill  

PROVIDER: MSV000092041 | MassIVE | Fri May 26 10:37:00 BST 2023

REPOSITORIES: MassIVE