Dataset Information

Fluorinated ethylamines as electrospray-compatible neutral pH buffers for native mass spectrometry

ABSTRACT: All filenames have been associated with the corresponding manuscript figure below: Figure 2: BD_1.raw, BD_2.raw, BD_3.raw, BD_4.raw, BD_5.raw, BD_6.raw, BD_10.raw, BD_11.raw, BD_12.raw Figure 3: BD_74.raw, BD_76.raw, BD_79.raw Figure 4: BD_242A.raw, BD_243A.raw, BD_246A.raw Figure S2: BD_7.raw, BD_8.raw, BD_9.raw, BD_13.raw,BD_14.raw,BD_15.raw Figure S3: BD_72.raw, BD_73.raw, BD_91.raw Figure S5: BD_132.raw, BD_133.raw, BD_134.raw, BD_135.raw, BD_136.raw, BD_137.raw, BD_138.raw, BD_140.raw, BD_141.raw, BD_142.raw, BD_143.raw, BD_144.raw, BD_145.raw, BD_146.raw, BD_147.raw, BD_148.raw, BD_149.raw, BD_150.raw, BD_151.raw, BD_152.raw, BD_153.raw, BD_154.raw, BD_155.raw, BD_156.raw, BD_157.raw, BD_158.raw, BD_159.raw, BD_160.raw, BD_168.raw, BD_169.raw, BD_170.raw, BD_171.raw, BD_172.raw, BD_173.raw, BD_174.raw, BD_175.raw, BD_176.raw, BD_177.raw, BD_178.raw, BD_179.raw, BD_180.raw, BD_181.raw, BD_182.raw, BD_183.raw, BD_184.raw, BD_185.raw, BD_186.raw, BD_187.raw, BD_188.raw, BD_189.raw, BD_190.raw, BD_191.raw, BD_192.raw, BD_193.raw Figure S7: SV_2674.raw, SV_2675.raw, SV_2676.raw Due to ongoing server issues, some of the listed files are not currently uploaded.

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Equus Caballus (ncbitaxon:9796) Bos Taurus (ncbitaxon:9913) Saccharomyces Cerevisiae (ncbitaxon:4932) Canavalia Ensiformis (ncbitaxon:3823)

SUBMITTER: Dr. Siavash Vahidi

PROVIDER: MSV000092212 | MassIVE | Mon Jun 19 14:02:00 BST 2023

REPOSITORIES: MassIVE

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Publications

Fluorinated Ethylamines as Electrospray-Compatible Neutral pH Buffers for Native Mass Spectrometry.

Davis Bradley T V BTV Velyvis Algirdas A Vahidi Siavash S

Analytical chemistry 20231124 48

Native electrospray ionization mass spectrometry (ESI-MS) has emerged as a potent tool for examining the native-like structures of macromolecular complexes. Despite its utility, the predominant "buffer" used, ammonium acetate (AmAc) with p<i>K</i><sub>a</sub> values of 4.75 for acetic acid and 9.25 for ammonium, provides very little buffering capacity within the physiological pH range of 7.0-7.4. ESI-induced redox reactions alter the pH of the liquid within the ESI capillary. This can result in ...[more]

PMID: 37997939

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