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Alzheimer's Disease Protein Relevance Analysis Using Human and Mouse Model Proteomics Data

ABSTRACT: Brain tissue was homogenized in 0.5% ALS-1 and protein extractions were reduced (10 mM DTT, 80C, 15 min) and alkylated (20 mM IAM, wt, 30 min) followed by digestion with 1:50 trypsin overnight at 37 degC. An SPQC pool was made by mixing an equal amount of digests. 0.75 ugs was analyzed by 1D-LC-MS/MS using a top 12 DDA method .

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Michael W. Lutz

PROVIDER: MSV000092255 | MassIVE | Fri Jun 23 13:46:00 BST 2023

REPOSITORIES: MassIVE

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