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Proteomics of exovesicle-enriched fraction of Norway Spruce, non-EDTA-extracted samples

ABSTRACT: Exovesicle-enriched fraction was isolated from the culture medium of tissue-cultured cells of Norway spruce during extracellular lignin formation and when lignin formation was hindered by scavenging of H2O2 from the culture medium. Proteomic analysis was conducted to investigate the protein cargo of the exovesicles.

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Picea Abies (ncbitaxon:3329)

SUBMITTER: Anna Happonen

PROVIDER: MSV000092312 | MassIVE | Fri Jun 30 02:48:00 BST 2023

SECONDARY ACCESSION(S): PXD043420

REPOSITORIES: MassIVE

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Extracellular vesicles of Norway spruce contain precursors and enzymes for lignin formation and salicylic acid.

Kankaanpää Santeri S Väisänen Enni E Goeminne Geert G Soliymani Rabah R Desmet Sandrien S Samoylenko Anatoliy A Vainio Seppo S Wingsle Gunnar G Boerjan Wout W Vanholme Ruben R Kärkönen Anna A

Plant physiology 20241001 2

Lignin is a phenolic polymer in plants that rigidifies the cell walls of water-conducting tracheary elements and support-providing fibers and stone cells. Different mechanisms have been suggested for the transport of lignin precursors to the site of lignification in the cell wall. Extracellular vesicle (EV)-enriched samples isolated from a lignin-forming cell suspension culture of Norway spruce (Picea abies L. Karst.) contained both phenolic metabolites and enzymes related to lignin biosynthesis ...[more]

PMID: 38771246

Similar Datasets

Proteomics of exovesicle-enriched fraction of Norway spruce

Project description:Exovesicle-enriched fraction was isolated from the culture medium of tissue-cultured cells of Norway spruce during extracellular lignin formation and when lignin formation was hindered by scavenging of H2O2 from the culture medium. Proteomic analysis was conducted to investigate the protein cargo of the exovesicles.

2023-05-16 | MSV000091958 | MassIVE

Proteomic analysis of unconcentrated exovesicle-depleted cell culture medium of Norway spruce

Project description:Exovesicle-enriched fraction was isolated from the culture medium of tissue-cultured cells of Norway spruce during extracellular lignin formation by an ultracentrifugation-based protocol. From the remaining, now exovesicle-depleted culture medium, 11.8 ul of the medium was prepared for proteomic analysis.

2023-12-18 | MSV000093681 | MassIVE

Down-regulation of a gene encoding CCR affects... cell wall function in embryogenic cultures of Norway spruce

Project description:An attractive objective in the breeding of forest trees is to reduce the content of lignin or alter the lignin composition. However, for long-lived forest trees, such as conifers, it is time consuming and expensive to wait until mature wood is formed for estimating how different ligninfication genes affect cell wall differentiation. The aim of this work was to examine the possibility of detecting changes in global gene expression and of genes involved in lignin biosynthesis already in embryogenic cultures carrying the native Norway spruce cinnamoyl CoA reductase (CCR) gene in antisense orientation. Several genes, including genes regulating phenylalanine ammonia-lyase and peroxidase, regulating different pathways associated with lignin biosynthesis were affected but the transcript abundances of genes regulating lignin biosynthesis were not affected. Functional assignment defined by Gene Ontology terms, which provide functional classification for genes and gene products representing their corresponding biological process, cellular component and molecular function showed that transformation with asCCR affects a broad spectrum of functional categories, especially processes taking place in the cell wall. Furthermore, many changes in genes related to chromatin, DNA and chromosome take place. Keywords: transgenic Norway spruce, embryogenic culture

2006-12-27 | GSE6524 | GEO

Project description:Apoplastic H2O2 effects on phenolic metabolism and gene expression in a Norway spruce lignin-forming cell culture

| PRJEB13079 | ENA

DNA methylome study of Norway spruce

Project description:Bisulfite treatment libraries were made to characterize DNA methylation of Norway spruce

2016-11-22 | GSE86983 | GEO

Hunting monolignol transporter(s): membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce

Project description:Monolignol transport during lignification is a partially solved puzzle: both the mechanism(s) and the transported form of monolignols are unknown in developing xylem of trees. We tested a hypothesis of an active, plasma membrane (PM)-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared of developing xylem, phloem, and lignin-forming, tissue-cultured cells of Norway spruce (Picea abies L. Karst.), as well as of a control material, non-lignifying tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported both coniferin and p-coumaryl alcohol glucoside, but inhibitor assays suggested this transport being over the tonoplast. Based on similar inhibitor assays, lignin-forming, tissue42 cultured cells of spruce had coniferin transport putatively localizing on PM. Liquid chromatography-mass spectrometry (LC-MS/MS) analysis of membrane proteins isolated from spruce developing xylem, phloem and tissue-cultured cells revealed multiple transporters. These were compared to a transporter gene set that was gained by a correlation analysis with a selected set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays showed no support for the ABC-transporter-mediated monolignol transport but point to secondary active transporters (such as MFS- or MATE-transporters). In contrast, proteomic and co-expression analyses suggest a role for ABC-transporters and MFS-transporters.

2020-08-14 | PXD017533 | Pride

PARE libraries for the identification of miRNA target genes in Norway spruce

Project description:We constructed four PARE libraries for the identification of miRNA target genes in Norway spruce.

2015-09-02 | GSE65248 | GEO

Norway Spruce Cultured Cells Chitosan & C.polonica Stress

Project description:Conifer-specific responses to elicitation with the chemical elicitor chitosan have been investigated using a transcriptome analysis in Norway spruce using a cell suspension culture system that has been previously described (Phillips, Walter et al. 2007). This study has demonstrated that the early events following chitosan elicitation include calcium mediated signaling and an oxidative response that have not previously been described in intact trees. Keywords: stress response

2008-06-01 | GSE10771 | GEO

Fine root gene expression of Norway spruce cuttings

Project description:We investigated root associated fungi in young Norway spruce (Picea abies) cuttings rooted from slow- and fast-growing trees showing variable growth rate in long-term field experiments and compared their roots’ gene expression patterns five and 18 months after adventitious root initiation. Gene expression patterns of adventitious roots could not be systematically linked with the growth phenotype at the initiation of root formation, and thus fundamental differences in the receptiveness of fungal symbionts could not be assumed.

2023-11-02 | GSE246281 | GEO

Nutrient limitation-induced regulation of Phanaerochaete chrysosporium genes

Project description:Transcript profiles of Phanerochaete chrysosporium grown on carbon-limited medium, nitrogen-limited medium and replete medium. Array design based on the DoE's Joint Genome Institute's v2.1 annotation. Goal is to define genes involved in lignin degradation. Keywords: gene expression under three different culture conditions

2009-03-30 | GSE14735 | GEO

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