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Proteomics Analysis of the UMK2 Mutation in Arabidopsis thaliana seeds

ABSTRACT: We compared the proteome of dry as well as germinating seeds of Arabidopsis thaliana wild type Col-0 with the respective proteomes from an umk2 (AT4G25280) frameshift mutant. Germinating seeds were incubated for 48 hours in 1/2-MS medium in Petri dishes at 4 degree Celsius followed by 24 hours of a phytochamber under long-day conditions. Protein extraction, digestion by SP3 workflow, and LC-IMS-MS/MS analysis as described in https://doi.org/10.1038/s41477-022-01308-6.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Hans-Peter Braun Nils Rugen

PROVIDER: MSV000092331 | MassIVE | Mon Jul 03 00:39:00 BST 2023

SECONDARY ACCESSION(S): PXD043459

REPOSITORIES: MassIVE

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Three Arabidopsis UMP kinases have different roles in pyrimidine nucleotide biosynthesis and (deoxy)CMP salvage.

Rinne Jannis J Niehaus Markus M Medina-Escobar Nieves N Straube Henryk H Schaarschmidt Frank F Rugen Nils N Braun Hans-Peter HP Herde Marco M Witte Claus-Peter CP

The Plant cell 20240901 9

Pyrimidine nucleotide monophosphate biosynthesis ends in the cytosol with uridine monophosphate (UMP). UMP phosphorylation to uridine diphosphate (UDP) by UMP KINASEs (UMKs) is required for the generation of all pyrimidine (deoxy)nucleoside triphosphates as building blocks for nucleic acids and central metabolites like UDP-glucose. The Arabidopsis (Arabidopsis thaliana) genome encodes five UMKs and three belong to the AMP KINASE (AMK)-like UMKs, which were characterized to elucidate their contri ...[more]

PMID: 38865437

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