Project description:To achieve extreme manganese stress, we used DmntP E. coli strain. We identified the pathways that are altered under manganese stress. Mainly, manganese altered the metabolism of iron in the cell. Therefore, we have shown that iron supplementation mitigates manganese toxicity. Overall, our data explains the basis of manganism in higher organisms.
Project description:To investigate acclimation mechanisms employed under extreme high light conditions, gene expression analysis was performed using the model microalgae Synechocystis sp. PCC 6803 (PCC 6803) cultured under various light intensities. From the low to the mid light conditions, the expression of genes related to light harvesting systems was repressed, whereas that of CO2 fixation and of D1 protein turnover-related genes was induced. Gene expression data also revealed that the down-regulation of genes related to flagellum synthesis (pilA2), pyridine nucleotide transhydrogenase (pntA and pntB), and sigma factor (sigA and sigF) represents acclimation mechanisms of PCC 6803 under excessive high light conditions.
Project description:Mahonia Bealei was used as a traditional Chinese medicine for its high alkaloid content. Previous research found that alkaloid and flavonoid contents in the M. bealei leaves increased under combinatory treatments of ultraviolet B and dark. In order to explore the underlying response mechanism, TiO2 material enrichment and mass-based label-free quantitative proteomics techniques were used for phosphoproteomics analysis of M. bealei leaves under ultraviolet B. ATP content, photosynthetic pigment content, and some enzymatic/non-enzymatic indicators increased in the leaves of M. bealei under UV-B radiation. Phosphoproteomics study found that under the UV-B radiation, phosphoproteins related to MAPK signal transduction and plant hormone brassinosteroid signaling pathway were varied greatly. Phosphoproteins related to photosynthesis, glycolysis, tricarboxylic acid cycle, and amino acid synthesis/metabolism pathway were also significantly changed. These results suggested that the ultraviolet B radiation activated oxidative stress system, MAPK signal transduction pathway, and photosynthetic energy metabolism pathway. These changes are important for the redox reactions in secondary metabolism and the accumulation of secondary metabolites in M. bealei leaves under UV-B radiation.
Project description:The molecular mechanisms by which individuals subjected to environmental heat stress either adapt or develop heat-related complications are not well understood. We analysed the changes in blood mononuclear gene expression patterns in human volunteers exposed to an extreme heat in a sauna (temperature of 78 ± 6 °C).
Project description:The molecular mechanisms by which individuals subjected to environmental heat stress either adapt or develop heat-related complications are not well understood. We analysed the changes in blood mononuclear gene expression patterns in human volunteers exposed to an extreme heat in a sauna (temperature of 78 ± 6 °C).