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Exposure to extracellular vesicles from Anisakis simplex induce changes in protein composition of human intestinal epithelial cell line (CACO-2) - parasite-host interaction overview

ABSTRACT: Anisakis simplex is one of the most prevalent parasitic nematodes (Nematoda) of marine organisms and is characterized by a complex life cycle. Humans can be accidental hosts for this parasitic species. Therefore, A. simplex was acknowledged as a biohazardous organism. The finding that nematodes can release extracellular vesicles (EVs), which are able to enter host cells, was the breakthrough discovery in parasite research. Although several approaches have been employed to study the biology of nematodes and their interactions with the host, the secretion of EVs by parasitic nematodes, as signal molecules, has been poorly studied. This led us to identify differentially regulated proteins (DRPs) between the proteome of a human intestinal epithelial cell line (CACO 2) exposed to EVs of A. simplex and the proteome of CACO 2 directly exposed to L3 larvae of A. simplex. In addition, we identified proteins present in EVs of A. simplex larvae and linked them to host proteins that they might regulate. To achieve this goal the shotgun proteomics method based on isobaric mass labeling (TMT) with a combination of nano high-performance liquid chromatography (nLC) coupled to an LTQ Orbitrap Elite mass spectrometer was used.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Anisakis Simplex (ncbitaxon:6269)

SUBMITTER: Robert Stryinski

PROVIDER: MSV000092443 | MassIVE | Fri Jul 14 04:55:00 BST 2023

REPOSITORIES: MassIVE

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